Effect of TEMPOL,l-NAME, apocynin, and ketotifen on jejunal weight, mucosal lesions, leukotriene generation, and NOS activity in iodoacetamide treated rats
| Treatment | None | TEMPOL | l-NAME | Apocynin | Ketotifen |
| No. of rats | 7–14 | 8–14 | 8 | 8–10 | 7 |
| Weight (g/25 cm) | 3.80 (0.25) | 4.0 (0.2) | 3.7 (0.4) | 2.40 (0.07)7-150 | 2.00 (0.07)7-150 |
| Lesion (mm2/rat) | 1684 (418) | 2551 (515) | 1322 (444) | 26.0 (5.0)7-150 | 5.0 (5.0)7-150 |
| Range | 420–6820 | 600–7200 | 0–3900 | 0–56 | 0–35 |
| Median | 1100 | 2035 | 1140 | 30 | 0 |
| NOS (nmol/g/min) | 16.90 (2.97) | 21.8 (2.7) | 18.50 (2.75) | 3.00 (0.26)7-150 | 3.90 (0.39)7-150 |
| LTB4 (ng/g) | 7.10 (0.45) | 3.2 (0.7)7-150 | 1.5 (0.5)7-150 | 5.60 (0.56) | 5.0 (1.0) |
| LTC4 (ng/g) | 6.60 (1.28) | 2.9 (0.4)7-150 | 3.10 (0.77)7-150 | 3.97 (0.95) | 8.0 (1.5) |
| PGE2 (ng/g) | 37.0 (6.2) | 28.3 (5.6) | 21.7 (5.7) | 49.5 (7.5)7-150 | 54.0 (5.0)7-150 |
Results are expressed as mean (SE).
Rats were injected intrajejunally with 0.1 ml iodoacetamide (2%) and co-treated daily intragastrically with TEMPOL (50 mg/100 g body weight) or ketotifen (200 μg body weight). Other groups were co-treated with l-NAME (0.1 mg/ml) or apocynin (120 μg/ml) added to the drinking water. The control group was treated with iodoacetamide only. Rats were sacrificed after seven days, the small intestine was isolated, the proximal 25 cm section was weighed, lesions were measured, and mucosal NOS activity, and production of LTB4, LTC4 and PGE2measured as described in Methods.
↵7-150 Significantly different from rats treated with iodoacetamide only (p<0.05).