Summary of ISH results
| Probe | Days after ulcer induction | Lamina propria | Submucosa | Muscularis propria | Serosa | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Contralateral | Ulcer | Contralateral | Ulcer | Contralateral | Ulcer | Contralateral | Ulcer | ||||||||||
| α1 (I) procollagen | 3 | +(+) | + | +++ | − | ++ | − | ++ | |||||||||
| 5 | − | +(+) | + | +++ | − | ++ | − | ++ | |||||||||
| 7 | − | ++ | + | +++ | − | ++(+) | − | ++ | |||||||||
| 12 | − | ++ | + | +++ | − | ++(+) | − | ++ | |||||||||
| 15 | − | + | − | +++ | − | ++ | − | + | |||||||||
| 30 | − | − | − | + | − | − | − | − | |||||||||
| α1 (III) procollagen | 3 | − | + | + | ++ | − | + | − | + | ||||||||
| 5 | − | + | + | ++ | − | + | − | + | |||||||||
| 7 | − | + | + | ++ | − | + | − | + | |||||||||
| 12 | − | + | + | ++ | − | + | − | + | |||||||||
| 15 | − | (+) | − | ++ | − | − | − | + | |||||||||
| 30 | − | − | − | − | − | − | − | − | |||||||||
| α1 (IV) procollagen | 3 | − | + | + | ++ | − | +(+) | − | + | ||||||||
| 5 | − | + | + | ++ | − | +(+) | − | + | |||||||||
| 7 | − | + | + | ++ | − | +(+) | − | + | |||||||||
| 12 | − | + | + | ++ | − | +(+) | − | + | |||||||||
| 15 | − | (+) | − | ++ | − | (+) | − | + | |||||||||
| 30 | − | − | − | + | − | − | − | − | |||||||||
−, +, ++, and +++ refer to the relative abundance of silver grains over individual cells after hybridisation with α1(I), α1(II), and α1(IV) probes after six, 20, and 15 days of autoradiographic exposure, respectively, on the ulcer and contralateral sides: −, background signal; (+), 2–5 times; +, 5–10 times; ++, 10–15 times; and +++, >15 times the background signal as defined by hybridisation with sense (control) probes.