IL-10 release | Proliferation | ||||||
---|---|---|---|---|---|---|---|
PBL-T | LPL-T | PBL-T | LPL-T | ||||
Experiment 1 | |||||||
Medium | < Min | < Min | 59 | 89 | |||
T112/3 + SRBC | 1068 | 2176 | 22 872 | 34 562 | |||
T112/3 | < Min | 488 | 7 235 | 32 882 | |||
OKT-3 + PBMO | 27 | 796 | 9 316 | 47 998 | |||
OKT-3 + IL-2 | 48 | < Min | 30 030 | 1 918 | |||
PBMO | < Min | < Min | 377 | 250 | |||
OKT-3 | < Min | < Min | 265 | 52 | |||
Experiment 2 | |||||||
Medium | < Min | < Min | 169 | 46 | |||
T112/3 + SRBC | 744 | 1949 | 40 819 | 58 978 | |||
T112/3 | < Min | 248 | 904 | 28 984 | |||
OKT-3 + PBMO | 98 | 764 | 2 560 | 13 422 | |||
OKT-3 + IL-2 | 54 | 118 | 46 987 | 4 392 | |||
Experiment 3 | |||||||
Medium | < Min | < Min | 100 | 44 | |||
T112/3 + SRBC | 566 | 1918 | 52 888 | 48 306 | |||
T112/3 | < Min | 317 | 890 | 25 307 | |||
OKT-3 + PBMO | 40 | 1460 | 11 670 | 55 503 | |||
OKT-3 + IL-2 | 72 | 34 | 37 266 | 2 367 |
PBL-T and LPL-T were activated through various stimuli: CD2 monoclonal antibodies T112 and T113 (1/3000 v/v) in the presence or absence of SRBC (30 μl 5% SRBC for 106 cells), Sepharose bound CD3 monoclonal antibody OKT-3 (1/100 v/v) in the presence or absence of IL-2 (10 U/ml) or autologous irradiated (50 Gy) PBMO (30% of total cell number) and PBMO only. Supernatants were harvested after 60 hours and the IL-10 content of each (results expressed in pg/ml; detection limit (min) 10 pg/ml) was determined by using ELISA. Cell growth was measured by the incorporation of [3H]-thymidine after four days. Results are expressed as the mean cpm of triplicate cultures. SD < 20%.