IL-10 release | Proliferation | ||||||
---|---|---|---|---|---|---|---|
Unseparated | CD45RA+ | CD45RO+ | Unseparated | CD45RA+ | CD45RO+ | ||
Experiment 1 | |||||||
Medium | < Min | < Min | < Min | 66 | 124 | 46 | |
T112/3 + SRBC | 702 | < Min | 1369 | 59 361 | 16 472 | 25 698 | |
T112/3 | < Min | < Min | 11 | 382 | 357 | 112 | |
OKT-3 + PBMO | 138 | 20 | 234 | 44 457 | 23 313 | 31 403 | |
OKT-3 + IL-2 | 216 | < Min | 478 | 46 908 | 23 510 | 37 445 | |
PBMO | 19 | 20 | 18 | 624 | 165 | 657 | |
OKT-3 | < Min | < Min | < Min | 380 | 135 | 62 | |
Experiment 2 | |||||||
Medium | < Min | < Min | < Min | 49 | 56 | 35 | |
T112/3+ SRBC | 1937 | < Min | 1333 | 66 749 | 9 937 | 29 736 | |
T112/3 | < Min | < Min | < Min | 562 | 1 172 | 48 | |
OKT-3 + PBMO | 164 | 45 | 257 | 37 833 | 22 259 | 38 923 | |
OKT-3 + IL-2 | 132 | 12 | 133 | 53 720 | 53 936 | 51 918 | |
PBMO | 26 | < Min | 23 | 1 081 | 828 | 2 145 | |
Experiment 3 | |||||||
Medium | < Min | < Min | < Min | 39 | 53 | 38 | |
T112/3+ SRBC | 1209 | < Min | 1786 | 30 090 | 5 613 | 15 197 | |
T112/3 | < Min | < Min | < Min | 698 | 2 832 | 132 | |
OKT-3 + PBMO | 286 | 40 | 546 | 50 945 | 16 681 | 50 745 | |
OKT-3 + IL-2 | 162 | < Min | 291 | 45 265 | 13 797 | 13 771 |
Unseparated and CD45RA+ and CD45RO+ PBL-T, respectively, were stimulated by CD2 monoclonal antibodies T112 and T113 (1/3000 v/v) in the presence or absence of SRBC (30 μl 5% SRBC for 106 cells), Sepharose bound CD3 monoclonal antibody OKT-3 (1/100 v/v) in the presence or absence of IL-2 (10 U/ml) or autologous irradiated (50 Gy) PBMO (30% of total cell number) and PBMO only. Supernatants were harvested after 60 hours and the IL-10 content of each (results expressed in pg/ml; detection limit (min) 10 pg/ml) was determined by using ELISA. Cell growth was measured by the incorporation of [3H]-thymidine after four days. Results are expressed as the mean cpm of triplicate cultures. SD < 20%.