Specifications of the primer sets used to analyse cytokine mRNA expression
Cytokine | Primer set | Annealing temperature | PCR product size (bp) |
β-actin | 5′-TGACGGGGTCACCCACACTGTGCCCATCTA-3′ | 72 | 661 |
5′-CTAGAAGCATTGCGGTGGACGATGGAGGG-3′ | |||
IL-1α | 5′-GTCTCTGAATCAGAAATCCTTCTATC-3′ | 60 | 421 |
5′-CATGTCAAATTTCACTGCTTCATCC-3′ | |||
IL-1β | 5′-AAACAGATGAAGTGCTCCTTCCAGG-3′ | 60 | 391 |
5′-TGGAGAACACCACTTGTTGCTCCA-3′ | |||
IL-2 | 5′-GAATGGAATTAATAATTACAAGAATCCC-3′ | 60 | 222 |
5′-TGTTTCAGATCCCTTTAGTTCCAG-3′ | |||
IL-3 | 5′-TCCAAACATGAGCCGCCTGCC-3′ | 60 | 211 |
5′-CATCAGAATGTCTTGGTCTTC-3′ | |||
IL-4 | 5′-AACACAACTGAGAAGGAAACCTTC-3′ | 65 | 276 |
5′-GCTCGAACACTTTGAATATTTCTC-3′ | |||
IL-5 | 5′-GCTTCTGCATTTGAGTTTGCTAGCT-3′ | 65 | 293 |
5′-TGGCCGTCAATGTATTTCTTTATTAAG-3′ | |||
IL-6 | 5′-ATGAACTCCTTCTCCACAAGCGC-3′ | 60 | 628 |
5′-GAAGAGCCCTCAGGCTGGACTG-3′ | |||
IL-7 | 5′-ATGTTCCATGTTTCTTTTAGGTATATCT-3′ | 60 | 680 |
5′-TGCATTTCTCAAATGCCCTAATCCG-3′ | |||
IL-8 | 5′-ATGACTTCCAAGCTGGCCGTGGCT-3′ | 60 | 289 |
5′-TCTCAGCCCTCTTCAAAAACTTCTC-3′ | |||
IL-9 | 5′-TCACCATGGTTCTGGCCATGGTCCTTACCTC-3′ | 60 | 487 |
5′-ATAATATTTCATCTTCATATCTTGCCTCTCA-3′ | |||
IL-10 | 5′-ATGCCCCAAGCTGAGAACCAAGACCCA-3′ | 72 | 352 |
5′-TCTCAAGGGGCTGGGTCAGCTATCCCA-3′ | |||
IL-11 | 5′-ACTGCTGCTGCTGAAGACTCGGCTGTGA-3′ | 62 | 322 |
5′-ATGGGGAAGAGCCAGGGCAGAAGTCTGT-3′ | |||
IL-12p40 | 5′-AGTGTCAAAAGCAGCAGAGG-3′ | 60 | 363 |
5′-AACGCAGAATGTCAGGGAG-3′ | |||
IL-13 | 5′-GCATGGTATGGAGCATCAAC-3′ | 60 | 371 |
5′-ATTTTACCCCTCCCTAACCCT-3′ | |||
IL-14 | 5′-CCCTCCAGTTCTTTATCC-3′ | 55 | 224 |
5′-CAACAGCTTGCCCTATAC-3′ | |||
IL-15 | 5′-TGGAGCACAGAAATCAATG-3′ | 55 | 265 |
5′-GAAACAGCCCAAAATGAAG-3′ | |||
IFN-γ | 5′-ATGAAATATACAAGTTATATCTTGGCTTT-3′ | 60 | 501 |
5′-GATGCTCTTCGACCTCGAAACAGCAT-3′ | |||
TNF-α | 5′-CGGGACGTGGAGCTGGCCGAGGAG-3′ | 72 | 355 |
5′-CACCAGCTGGTTATCTCTCAGCTC-3′ | |||
TGF-β1 | 5′-GCCCTGGACACCAACTATTGCT-3′ | 65 | 161 |
5′-AGGCTCCAAATGTAGGGGCAGG-3′ | |||
GM-CSF | 5′-GAGCATGTGAATGCCATCCAGGAG-3′ | 55 | 390 |
5′-CTCCTGGACTGGCTCCCAGCAGTCAAA-3′ |