Production | None | IL-1β (100 U/ml) | TNF-α (100 U/ml) | ||||||
---|---|---|---|---|---|---|---|---|---|

6 h | 24 h | 6 h | 24 h | ||||||

Bioactive IL-1
| |||||||||

Exp 1 SN | Negative | ND | ND | 0.5 U/ml | 0.5 U/ml | ||||

Exp 1 CL | Negative | ND | ND | 2 U/ml | 1 U/ml | ||||

Exp 2 SN | Negative | ND | ND | Negative | 0.5 U/ml | ||||

Exp 2 CL | Negative | ND | ND | 1 U/ml | 1 U/ml | ||||

Exp 3 SN | Negative | ND | ND | Negative | Negative | ||||

Exp 3 CL | Negative | ND | ND | 15 U/ml | 30 U/ml | ||||

Immunoreactive IL-1α
| |||||||||

Exp 2 SN | ND | ND | ND | ND | ND | ||||

Exp 2 CL | Negative | 15 pg/ml | 10 pg/ml | 8 pg/ml | 8 pg/ml | ||||

Exp 3 SN | Negative | ND | ND | Negative | Negative | ||||

Exp 3 CL | Negative | ND | ND | 14 pg/ml | 30 pg/ml |

SN, supernatant; CL, cell lysate; ND, not determined.