Measurement of IL-1 protein in HIMEC cultures before and after cytokine stimulation
| Production | None | IL-1β (100 U/ml) | TNF-α (100 U/ml) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 6 h | 24 h | 6 h | 24 h | ||||||
| Bioactive IL-1 | |||||||||
| Exp 1 SN | Negative | ND | ND | 0.5 U/ml | 0.5 U/ml | ||||
| Exp 1 CL | Negative | ND | ND | 2 U/ml | 1 U/ml | ||||
| Exp 2 SN | Negative | ND | ND | Negative | 0.5 U/ml | ||||
| Exp 2 CL | Negative | ND | ND | 1 U/ml | 1 U/ml | ||||
| Exp 3 SN | Negative | ND | ND | Negative | Negative | ||||
| Exp 3 CL | Negative | ND | ND | 15 U/ml | 30 U/ml | ||||
| Immunoreactive IL-1α | |||||||||
| Exp 2 SN | ND | ND | ND | ND | ND | ||||
| Exp 2 CL | Negative | 15 pg/ml | 10 pg/ml | 8 pg/ml | 8 pg/ml | ||||
| Exp 3 SN | Negative | ND | ND | Negative | Negative | ||||
| Exp 3 CL | Negative | ND | ND | 14 pg/ml | 30 pg/ml | ||||
SN, supernatant; CL, cell lysate; ND, not determined.