Table 3

Cytokine production profile of one HUVEC culture before and after cytokine stimulation

ProductionNoneIL-1β (100 U/ml)TNF-α (100 U/ml)
6 h24 h6 h24 h
Bioactive IL-1
Exp 1 SNNegativeNDND0.5 U/ml1 U/ml
Exp 1 CLNegativeNDND15 U/ml20 U/ml
Immunoreactive IL-1a
Exp 1 SN IL-1NegativeNDNDNegativeNegative
Exp 1 CL IL-1NegativeNDND15 pg/ml35 pg/ml
Bioactive IL-6
Exp 1 SNNegative24 U/ml32 U/ml3 U/ml16 U/ml
Exp 1 CLNegative3 U/ml4 U/ml2 U/ml3 U/ml
Immunoreactive IL-8
Exp 1 SN1.6 ng/ml26 ng/ ml50 ng/ml12 ng/ml36 ng/ml
Exp 1 CL0.5 ng/ml8 ng/ml12 ng/ml3 ng/ml4 ng/ml
  • SN, supernatant; CL, cell lysate; ND, not determined.