Cytokine production profile of one HUVEC culture before and after cytokine stimulation
| Production | None | IL-1β (100 U/ml) | TNF-α (100 U/ml) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 6 h | 24 h | 6 h | 24 h | ||||||
| Bioactive IL-1 | |||||||||
| Exp 1 SN | Negative | ND | ND | 0.5 U/ml | 1 U/ml | ||||
| Exp 1 CL | Negative | ND | ND | 15 U/ml | 20 U/ml | ||||
| Immunoreactive IL-1a | |||||||||
| Exp 1 SN IL-1 | Negative | ND | ND | Negative | Negative | ||||
| Exp 1 CL IL-1 | Negative | ND | ND | 15 pg/ml | 35 pg/ml | ||||
| Bioactive IL-6 | |||||||||
| Exp 1 SN | Negative | 24 U/ml | 32 U/ml | 3 U/ml | 16 U/ml | ||||
| Exp 1 CL | Negative | 3 U/ml | 4 U/ml | 2 U/ml | 3 U/ml | ||||
| Immunoreactive IL-8 | |||||||||
| Exp 1 SN | 1.6 ng/ml | 26 ng/ ml | 50 ng/ml | 12 ng/ml | 36 ng/ml | ||||
| Exp 1 CL | 0.5 ng/ml | 8 ng/ml | 12 ng/ml | 3 ng/ml | 4 ng/ml | ||||
SN, supernatant; CL, cell lysate; ND, not determined.