Table 4

Measurement of IL-6 and IL-8 protein in three HIMEC cultures before and after cytokine stimulation

ProductionNoneIL-1β (100 U/ml)TNF-α (100 U/ml)
6 h24 h6 h24 h
Bioactive IL-6
Exp 1 SNNegative13 U/ml13 U/ml20 U/ml20 U/ml
Exp 1 CLNegative13 U/ml13 U/rnl8 U/ml7 U/ml
Exp 2 SNNegative16 U/ml18 U/ml18 U/ml5 U/ml
Exp 2 CLNegative4 U/ml4 U/ml2 U/ml4 U/ml
Exp 3 SN1.5 U/mlNDND1.5 U/ml1.5 U/ml
Exp 3 CLNegativeNDND1.5 U/ml2.7 U/ml
Immunoreactive IL-8
Exp 1 SN10 ng/ml20 ng/ml50 ng/ml20 ng/ml35 ng/ml
Exp 1 CL2 ng/ml6 ng/ml16 ng/ml4 ng/ml8 ng/ml
Exp 2 SN15 ng/ml30 ng/ml40 ng/ml24 ng/ml30 ng/rnl
Exp 2 CL1.6 ng/mlND12 ng/mlND7 ng/ml
Exp 3 SN10 ng/mlNDND10 ng/ml20 ng/ml
Exp 3 CL5 ng/mlNDND7.5 ng/rnl25 ng/ml
  • SN, supernatant; CL, cell lysate; ND, not determined.