Table 2

Effects of cycloheximide (CHX) on cytokine secreting cells

Cytokine CHX3-150 No of secreting cells/105 lymphocytes
Exp 1 Exp 2 Exp 3
IFN-γ122711361700
+87310001500
Exp 4 Exp 5 Exp 6
IL-420002358607
+14742300369
  • 3-150 Cell suspensions were preincubated for four hours at 37°C in the absence or presence of CHX (100 μg/ml), before being processed in the ELISPOT plates.