Table 3

Cytokine secretion by intestinal lymphocytes in response to in vitro polyclonal stimulation

No of IFN-γ secreting cells/105 lymphocytes
Preincubation None Medium PMA-ionophore None Medium PMA-ionophore
Exp 172401384437012699
Exp 22360129356106571
Exp 36201356454252943843
  • IFN-γ secretion was analysed by processing the cells directly in the ELISPOT plates or by preincubating the cells for four hours at 37°C in medium alone or in the presence of PMA-ionophore.