Cytokine secretion by intestinal lymphocytes in response to in vitro polyclonal stimulation
| No of IFN-γ secreting cells/105 lymphocytes | |||||||
| IEL | LPL | ||||||
| Preincubation | None | Medium | PMA-ionophore | None | Medium | PMA-ionophore | |
| Exp 1 | 724 | 0 | 1384 | 437 | 0 | 12699 | |
| Exp 2 | 236 | 0 | 12935 | 61 | 0 | 6571 | |
| Exp 3 | 620 | 135 | 6454 | 252 | 94 | 3843 | |
IFN-γ secretion was analysed by processing the cells directly in the ELISPOT plates or by preincubating the cells for four hours at 37°C in medium alone or in the presence of PMA-ionophore.