Table 1

Details of reaction mixtures used for HFE genotyping

Reaction number	Sense primer number	Sense primer sequence (5′–3′)	Nucleotide position	Antisense primer number	Antisense primer sequence (5′–3′)	Nucleotide position	Specificity	Product size (bp)
1	805	AGCTGTTCGTGTTCTATGATC	388–408	807	CTGGGTGCTCCACCTGGC	1066–1083	63H–282C	1460
2	805	AGCTGTTCGTGTTCTATGATC	388–408	808	CTGGGTGCTCCACCTGGT	1066–1083	63H–282Y	1460
3	806	AGCTGTTCGTGTTCTATGATG	388–408	807	CTGGGTGCTCCACCTGGC	1066–1083	63D–282C	1460
4	806	AGCTGTTCGTGTTCTATGATG	388–408	808	CTGGGTGCTCCACCTGGT	1066–1083	63D–282Y	1460
5	805	AGCTGTTCGTGTTCTATGATC	388–408	835	CTGTGGTTGTGATTTTCCATAA	535–566	63H	178
6	806	AGCTGTTCGTGTTCTATGATG	388–408	835	CTGTGGTTGTGATTTTCCATAA	535–566	63D	178
7	834	TTGGTGAAGGTGACACATCAT	846–866	807	CTGGGTGCTCCACCTGGC	1066–1083	282C	237
8	834	TTGGTGAAGGTGACACATCAT	846–866	808	CTGGGTGCTCCACCTGGT	1066–1083	282Y	237
Control primers (reactions 1–4)
	725	GCCTTCCCAACCATTCCCTT		726	TCACGGATTTCTGTTGTGTTTC		HGH control	425

Specificity of primers is determined by the underlined 3′ nucleotide. Primers 834 and 835 are consensus, non-allele specific primers. Nucleotides are numbered according to Feder et al¹ (Genbank accession number U60319). The genomic sequence of HFE has not been published, but from the known sequence of HLA-A, codons 63 and 282 would lie in exons 2 and 4, respectively, separated by about 1460 nucleotides. Examples of reactions 1–4 only are given in fig 1.