Regulation of TNF-α, IL-1β and IL-1ra secretion by IL-10 in PMN from patients with IBD and normal controls
| TNF-α | IL-1β | IL-1ra | |||||||||
| IL-10 (U/ml) | 0 | 10 | 100 | 0 | 10 | 100 | 0 | 10 | 100 | ||
| Normal controls (n=7–10) | 112 | 92‡ | 27* | 251 | 122* | 20† | 1272 | 1672* | 1888* | ||
| 16–222 | 27–135 | 12–32 | 56–320 | 54–132 | 12–32 | 1221–2039 | 1514–2412 | 1712–2378 | |||
| Crohn’s disease (n=8–11) | 252 | 135** | 26** | 598 | 312** | 32** | 1085 | 1732* | 2079** | ||
| 119–795 | 24–332 | 25–34 | 583–720 | 273–332 | 18–72 | 612–1481 | 1532–2667 | 1808–2782 | |||
| Ulcerative colitis (n=7–11) | 212 | 131* | 36** | 474 | 272** | 51** | 1151 | 1773* | 2032* | ||
| 143–385 | 108–147 | 22–45 | 372–582 | 178–278 | 32–52 | 979–1988 | 1562–2732 | 1712–2345 |
Values are expressed as median (interquartile range) because the data did not follow a normal distribution. PMN were stimulated with LPS (10 μg/ml) to assess TNF-α and IL-1β release. IL-1ra release was assessed in non-stimulated PMN. Regulation of cytokine secretion by IL-10 reached statistical significance in each group (Wilcoxon matched pairs analysis versus untreated control cells: *p⩽0.05, **p⩽0.01,†p⩽0.005, ‡not significant) whereas no statistical differences in the relative capacity of regulation were seen between PMN from normal controls and patients with Crohn’s disease or ulcerative colitis.