Interferon γ (IFN-γ) release in both unstimulated and phytohaemagglutinin (PHA) preactivated lamina propria T lymphocytes (LPLs) and autologous peripheral blood lymphocytes (PBLs) after 24 hours of culture
| IFN-γ secretion (pg/ml) | ||||
|---|---|---|---|---|
| Stimulus | Unstimulated LPLs | PHA stimulated LPLs | Unstimulated PBLs | PHA stimulated PBLs |
| Medium alone | 0 | 16 (4.1) | 0 | 23 (3.0) |
| Anti-CD2 antibody | 430 (40) | 500 (40) | 320 (10) | 2500 (84) |
| Anti-CD28 antibody | 28 (3.5) | 30 (5) | 20 (7.0) | 34 (2.2) |
Results are expressed as mean (SD) from six representative experiments.
Cells were cultured with or without the initial addition of anti-CD2 (1:1000 final dilution) or anti-CD28 antibody (at a final concentration 1 μg/ml).