Table 1

Effect of acetic acid and trinitrobenzenesulphonic acid (TNB) on rat colon

Treatment Control Acetic acid (1 day) TNB
1 day 7 days
No of rats12–2212–1614–2112–14
Weight (g/10 cm)0.61 (0.01)1.61 (0.07)* 1.80 (0.15)* 3.7 (0.4)*
MPO (U/g)0.98 (0.09)5.6 (0.9)* 6.9 (0.9)* 4.6 (0.9)*
NOS (nmol/g/min) + Ca2+ 2.93 (0.17)7.1 (1.1)* 11.8 (2.0)* 4.8 (0.3)*
NOS (nmol/g/min) − Ca2+ 2.06 (0.10)7.0 (1.1)* 12.0 (2.7)* 4.7 (0.3)*
PGE2 (ng/g)39.00 (1.76)48.0 (2.4)* 48.0 (2.5)* 48.0 (2.3)*
LTB4 (ng/g)1.0 (0.1)4.2 (1.0)* 2.5 (0.3)* 1.5 (0.1)
  • Results are expressed as mean (SEM).

  • Colitis was induced with acetic acid or TNB, as described in Materials and Methods. Rats were killed after one or seven days as indicated. The distal 10 cm long colonic segment was isolated and the mucosa was scraped for determination of eicosanoid generation, and myeloperoxidase (MPO) and nitric oxide synthase (NOS) activities.

  • Significantly different from control rats, *p<0.05.

  • PGE2, prostaglandin E2; LTB4, leukotriene B4.