Table 3

 Effect of hyperbaric oxygen (HBO) on colonic mucosal eicosanoid generation, and myeloperoxidase (MPO) and nitric oxide synthase (NOS) activities in acetic acid treated rats

Treatment Acetic acid Acetic acid + HBO
No of rats12–168–10
MPO (U/g)5.6 (0.9)3.2 (0.3)*
NOS (nmol/g/min) + Ca2+ 7.1 (1.1)4.0 (0.4)*
NOS (nmol/g/min) − Ca2+ 7.0 (1.1)3.3 (0.3)*
PGE2 (ng/g)48.0 (2.4)26.8 (3.1)*
LTB4 (ng/g)4.2 (1.0)2.2 (0.4)*
  • Results are expressed as mean (SEM).

  • Colitis was induced with acetic acid and followed with HBO treatment as described in Materials and Methods. Rats were killed after 24 hours. The distal 10 cm long colonic segment was isolated and the mucosa was scraped for determination of eicosanoid generation, and MPO and NOS activities.

  • Significantly different from acetic acid only, *p<0.05.

  • PGE2, prostaglandin E2; LTB4, leukotriene B4.