Table 4

 Effect of hyperbaric oxygen (HBO) on colonic mucosal eicosanoid generation, and myeloperoxidase (MPO) and nitric oxide synthase (NOS) activities in trinitrobenzene- sulphonic acid (TNB) treated rats

TNB TNB + HBO
Treatment 1 day 7 days 1 day 7 days
No of rats14–2112–14810
MPO (U/g)6.9 (0.9)4.6 (0.9)4.0 (0.7)1.4 (0.2)*
NOS (nmol/g/min) + Ca2+ 11.8 (2.0)4.8 (0.3)4.2 (0.1)* 2.7 (0.3)*
NOS (nmol/g/min) − Ca2+ 12.0 (2.7)4.7 (0.3)3.1 (0.3)* 2.4 (0.2)*
PGE2(ng/g)48.0 (2.5)48.0 (2.3)21.8 (2.7)* 28.9 (2.4)*
LTB4 (ng/g)2.5 (0.3)1.5 (0.1)1.8 (0.3)1.4 (0.2)
  • Results are expressed as mean (SEM).

  • Colitis was induced with TNB and rats were exposed to HBO as described in Materials and Methods. Rats were killed after one or seven days of treatment as indicated. The distal 10 cm long colonic segment was isolated and the mucosa was scraped for determination of eicosanoid generation, and MPO and NOS activities.

  • Significantly different from TNB only, *p<0.05.

  • PGE2, prostaglandin E2; LTB4, leukotriene B4.