Table 1

Primer sets for PCR and cycle sequencing

Exon numberFirst PCRSecond PCRSequencing
PlusMinusTemp (°C)PlusMinusTemp (°C)PlusMinusTemp (°C)
 11U31L3571U31L350
 22U12L1552U22L250
 3U306L119764U329L92450
 44U14L1574U24L2574U24L250
 54U25L2575U25L2575U25L250
  • See table 2 for DNA sequence of each primer.