Oligonucleotide primers and PCR conditions used for detection of mRNA of the prostaglandin E2 (PGE2) receptor subtypes EP1R, EP2R, EP3R and EP4R and SGLT1 in rat small intestinal enterocytes
| Oligonucleotide sequence (5’→3’) | Receptor and primer position (GenBank accession no) | Temperature profile |
|---|---|---|
| GTGGTGCTGCCAACAGGCGATAATGGCACA | mEP1R, 150–179, sense (D16338) | 1 min 95°C, 1 min 55°C, 2 min 72°C |
| GGGACCTGCGGTCTTTCGGAATCGTCGAGA | mEP1R, 792–821, antisense (D16338) | 1 min 95°C, 1 min 55°C, 2 min 72°C |
| CATGGCCCTGGAACGCTACC | rEP2R, 498–517, sense (U48858) | 1 min 95°C, 1 min 58°C, 2 min 72°C |
| TCAGTGAAGTCCGACAACAGAGG | rEP2R, 1095–1117, antisense (U48858) | 1 min 95°C, 1 min 58°C, 2 min 72°C |
| GCGGCGGGCGATGGAGGAGAG | rEP3R, 27–47, sense (X83855) | 1 min 95°C, 1 min 65°C, 2 min 72°C |
| GCGGGACACCAGGGCTTTGATG | rEP3R, 834–855, antisense (X83855) | 1 min 95°C, 1 min 65°C, 2 min 72°C |
| TCTCTTACATGTACGCGGGCTTCA | rEP4R, 731–754, sense (D28860) | 1 min 95°C, 1 min 60°C, 2 min 72°C |
| GTCTGGCAGGTATAGGAGGGTGTG | rEP4R, 1366–1389, antisense (D28860) | 1 min 95°C, 1 min 60°C, 2 min 72°C |
| CGGGCAGCTCTTTGACTACAT CCA | rSGLT1, 1516–1539, sense (D16101) | 1 min 95°C, 1 min 60°C, 2 min 72°C |
| AAGGCGGGGTTCAGGCAAAATA | rSGLT1, 2156–2177, antisense (D16101) | 1 min 95°C, 1 min 60°C, 2 min 72°C |
r, rat; m, mouse.