Effect of cAMP analogues on cell viability in DLD-1 cells exposed for 24 hours to cytomix (CM)
| Cell treatment | Viable cells (106/ml) | MTT assay (%) |
|---|---|---|
| Control | 0.254 (0.023) | 100 |
| CM | 0.246 (0.033) | 104.5 (2.7) |
| CM + 8Br-cAMP 0.1 mM | 0.203 (0.022)1-160 | 86.6 (4)1-160 |
| CM + 8Br-cAMP 1 mM | 0.151 (0.009)*** | 74.8 (4.8)*** |
| CM + Db-cAMP 0.1 mM | 0.213 (0.005)1-160 | 90.5 (5.1)1-160 |
| CM + Db-cAMP 1 mM | 0.185 (0.012)*** | 85.2 (6)*** |
Results expressed as mean (SEM) from at least three different experiments. The number of viable cells was assessed by the trypan blue exclusion test. Statistical significance was assessed by Student’st test.
↵1-160 p<0.01, ***p<0.001 compared with control.