Table 1

PCR HLA class II genotyping results

Case MethodHLA-DRB1HLA-DRB3, 4, 5HLA-DQB1Conclusion
1Dysplastic tissuenSSP04, 1353(4)1-151 NASame HLA type
Reference tissueSSP04, 1353(4)06, 08
2Neoplastic tissuenSSP0301, 1551(5), 52(3)NASame HLA type
Gastritis tissuenSSP0301, 1551(5), 52(3)NA
Gastrectomy tissueSSP0301, 1551(5), 52(3)02, 06
3Neoplastic tissuenSSP0301, 1452(3)NAContaminant
Reference tissuenSSP0301, 0452(3), 53(4)NA
4Neoplastic tissuenSSP0301, 0452(3), 53(4)NAContaminant
Reference (pb)SSP04, 0453(4)07, 08
Second patientnSSP04, 1053(4)NA
Third patientnSSP0701, 070153(4)NA
Fourth patient (pb)SSP12, 12No association0301/4, 0301/4
Fifth patient (pb)SSP0301, 1352(3)02, 06
Sixth patient (pb)SSP0701, 130252(3), 53(4)02, 06
Seventh patient (pb)SSP0301, 130252(3)02, 06
5Dysplastic tissuenSSP0103, 030152(3)NASame HLA type
Reference tissuenSSP0103, 030152(3)NA
6Malignant tissuenSSP03, 1152(3)NAContaminant
Reference tissueSSP01, 140252(3)05, 07
7Dysplastic tissuenSSP01, 0453(4)NAContaminant
Reference tissueSSP12, 130252(3)06, 07
8Dysplastic tissuenSSP0301, 1352(3)NAContaminant
Reference tissueSSP0301, 1452(3)021-152
9Dysplastic tissuenSSP01, 1551(5)NAContaminant
Reference tissueSSP15, 1551(5)06
Second patientSSP01, 1551(5)07, 08
10Neoplastic tissuenSSP0301, 1152(3)NAContaminant
Remaining tissuenSSP03, 1452(3)NA
  • SSP, direct PCR-SSP genotyping; nSSP, nested PCR-SSP genotyping; pb, peripheral blood derived DNA (all other results derived from formalin fixed, paraffin wax embedded DNA).

  • 1-151 The expected DRB3 (DR52) gene was not detected.

  • 1-152 The expected DQB1*05 allele was not detected.

  • In cases 1, 2, and 5, the chance of two unselected white Western individuals possessing the same HLA-DRB1 alleles is: case 1 (HLA-DRB1*04, 13) 6%; case 2 (HLA-DRB1*0301, 15) 8%; case 5 (HLA-DRB1*0103, 0301) <1%.4 ,11