Densitometric evaluation of MMP/actin as detected by western blots in samples from patients with and without pouchitis
MMP-1/actin | Active form of MMP-1/actin | MMP-2/actin | Active form of MMP-2/actin | |
---|---|---|---|---|
Pouchitis (n=5) | 77.67% (26.5–138.8) | 30.9% (12.1–60.5) | 69.6% (32.6–115.9) | 16.9% (10.1–25.3) |
Uninflamed pouch (n=5) | 50.6% (28.2–126.8) | 12.3% (1.9 44.8)2-150 | 23.4% (18.0–37.5)2-150 | 4.7% (2.5–6.8)2-150 |
Intensity of bands in western blots were quantified as described in material and methods.
Concentrations of MMPs are expressed as percentages in relation to optical actin bands (minimum and maximum values are indicated in parentheses).
↵2-150 p⩽0.05.