Polymerase chain reaction (PCR) for amplification of H pylori cagA, iceA, and vacA genes
| Region | Primer | Nucleotide sequence (5′→3′) | Size of PCR product (bp) | Reference |
|---|---|---|---|---|
| cagA | cagA-F | AATACACCAACGCCTCCAAG | 400 | 22 |
| cagA-R | TTGTTGCCGCTTTTGCTCTC | |||
| iceA1 | iceA1-F | GTGTTTTTAACCAAAGTATC | 246 | 9 |
| iceA1-R | CTATAGCCAGTCTCTTTGCA | |||
| iceA2 | iceA2-F | GTTGGGTATATCACAATTTAT | 229 or 334 | 9 |
| iceA2-R | TTGCCCTATTTTCTAGTAGGT | |||
| m1 | m1-F | GGTCAAAATGCGGTCATGG | 290 | 23 |
| m1-R | CCATTGGTACCTGTAGAAAC | |||
| m2 | m2-F | GGAGCCCCAGGAAACATTG | 352 | 23 |
| m2-R | CATAACTAGCGCCTTGCAC | |||
| m1T | m1T-F | GGCCACAATGCAGTCATGG | 290 | 6 |
| m1T-R | CTCTTAGTGCCTAAAGAAACA | |||
| m1Tm2 | m1T-F | GGCCACAATGCAGTCATGG | 300 | 6 |
| m2-R | CATAACTAGCGCCTTGCAC | |||
| s1a | s1a-F | GTCAGCATCACACCGCAAC | 190 | 23 |
| s-R | CTGCTTGAATGCGCCAAAC | |||
| s1b | s1b-F | AGCGCCATACCGCAAGAG | 187 | 23 |
| s-R | CTGCTTGAATGCGCCAAAC | |||
| s2 | s2-F | GCTTAACACGCCAAATGATCC | 199 | 23 |
| s-R | CTGCTTGAATGCGCCAAAC |