Monoclonal antibodies (mAb) used in this study
| Marker | mAb/clone | Isotype | Main reactivity | Source |
| TcR-αβ | βF1/8A3 | IgG1 | Monomorphic determinant of the β chain of TcR-αβ | T cell Diagnostics, Cambridge, MA, USA |
| TcR-αβ | αF1/3A8 | IgG2a | Monomorphic determinant of the α chain of TcR-αβ | T cell Diagnostics |
| TcR-αβ | BMA031 | IgG2b | Monomorphic determinant on the TcR-αβ complex | T cell Diagnostics |
| TcR-γδ | TCRδ1/5.A6.E9 | IgG1 | Monomorphic determinant of the δ chain of TcR-γδ | T cell Diagnostics |
| TcR-γδ | Immu510 | IgG1 | Monomorphic determinant of the δ chain of TcR-γδ | Immunotech, Marseilles, France |
| TcR-γδ | δTCS1/TS-1 | IgG1 | Vδ1-Jδ1/Jδ2 encoded determinant | T cell Diagnostics |
| TcR-γδ | δV1(a)/TS8 | IgG1 | Vδ1 encoded determinant | T cell Diagnostics |
| TcR-γδ | 15D | IgG1 | Vδ2 encoded determinant | T cell Diagnostics |
| TcR-γδ | P11.5B | IgG1 | Vδ3 encoded determinant | Immunotech |
| CD3 | UCHT1 | IgG1 | T cells and subsets of thymocytes | Dakopatts, Glostrup, Denmark |
| CD3 | SK7 | IgG1 | T cells and subsets of thymocytes | Becton-Dickinson, Mountain View, CA, USA |
| CD4 | MT310 | IgG1 | MHC class II restricted T cells | Dakopatts |
| CD8 | DK25 | IgG1 | MHC class I restricted T cells | Dakopatts |
| CD19 | HD37 | IgG1 | B cells | Dakopatts |
| CD20 | L-26 | IgG2a | B cells | Dakopatts |
| CD22 | 4KB128 | IgG2b | B cells | Dakopatts |
| CD14 | MΦP6 | IgG2b | Monocytes/macrophages | Becton-Dickinson |
| CD15 | C3D-1 | IgM | Granulocytes | Dakopatts |
| CD68 | EBM11 | IgG1 | Tissue macrophages, cytolytic lymphocytes | Dakopatts |
| CD57 | NC-1 | IgM | NK cells and subsets of T cells | Immunotech |
| CD1a | NA1/34 | IgG2a | Thymocytes, Langerhans cells, interdigitating cells | Dakopatts |
| CD45 | 2B11 and PD7/26 | IgG1 | Leucocytes | Dakopatts |
| CD45RO | UCHL-1 | IgG2a | Activated and primed T cells, thymocytes | Dakopatts |
| CD45RA | L28 | IgG1 | Resting and naive T cells | Becton-Dickinson |
| CD103 | HML-1/2G51 | IgG2a | α chain of the αEβ7 integrin, mucosal lymphocytes | Immunotech |
| CD28 | CD28.2 | IgG1 | Subpopulations of T cells | Immunotech |
| CD80 | BB-1 | IgM | Antigen presenting cells | Serotec, Oxford, UK |
| HLA-DR | DK22 | IgG2a | Monomorphic determinant of HLA-DR | Dakopatts |
| HLA-DQ | SPVL3 | IgG2a | Monomorphic determinant of HLA-DQ | Immunotech |
| IgM | R1/69 | IgG1 | Human IgM | Dakopatts |
| IgG | TM15 | IgG1 | Human IgG | The Binding site, Birmingham, UK |
| IgA | 6E2C1 | IgG1 | Human IgA | Dakopatts |
| bcl-2 | 124 | IgG1 | Antiapoptosis protein | Dakopatts |
| Epithelial antigen | BerEP4 | IgG1 | Human epithelial cells | Dakopatts |
| Follicular dendritic cells | Ki-M4 | IgG2a | Follicular dendritic cells (accesory B cell macrophages) in lymphoid organs | BMA Biomedicals AG, Augst, Switzerland |
| Follicular dendritic cells | CNA.42 | IgM | Non-lineage restricted antigen expressed on follicular dendritic cells | Dakopatts |
| Follicular dendritic cells | R4/23 (DRC-1) | IgM | Dendritic reticulum cells present in lymphoid follicles | Dakopatts |
| Negative control | DAK-G01 | IgG1 | A nigerglucose oxidase | Dakopatts |
| Negative control | DAK-G05 | IgG2a | A nigerglucose oxidase | Dakopatts |
| Negative control | DAK-G09 | IgG2b | A nigerglucose oxidase | Dakopatts |
| Negative control | DAK-G08 | IgM | A niger glucose oxidase | Dakopatts |