Treatment | Viable S typhimurium (log CFU/ml) | |
Inoculum S typhimurium | 7.3 (0.2) | |
Control CA1-SCS | 2.0 (0.2)3-160 | |
Control F9-SCS | 2.2 (0.4)3-160 | |
Ammonium sulphate precipitation3-1 | CA1-SCS | 7.1 (0.5) |
Ammonium sulphate precipitation3-1 | F9-SCS | 6.8 (0.4) |
Chloroform-methanol extraction3-2 | CA1-SCS | 5.1 (0.2)3-160 |
Chloroform-methanol extraction3-2 | F9-SCS | 2.4 (0.3)3-160 |
Dialysis3-3 | CA1-SCS | 6.9 (0.4) |
Dialysis3 | F9-SCS | 6.9 (0.4) |
Experimental conditions are described in materials and methods.
Results are presented as mean(SEM) obtained from three experiments.
↵3-1 Activity in pellet obtained by ammonium sulphate precipitation.
↵3-2 Activity in the chloroform fraction after methanol-chloroform extraction.
↵3-3 Activity in the SCS after dialysis with Spectra/Por membrane (molecular weight cut off 3500).
Statistical analysis with Student's t test,
↵3-160 p<0.01 compared with inoculum.