Immunohistochemical expression of p63 and p53 in columnar lined oesophagus
| p63 expression | p53 expression | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| − | +/− | + | ++ | − | +/− | + | ++ | ||
| Oesophageal squamous mucosa | 0 | 0 | 9 | 1 | 10 | 0 | 0 | 0 | |
| Barrett's mucosa without IM | 4 | 2 | 4 | 0 | 10 | 0 | 0 | 0 | |
| Barrett's mucosa with IM | 4 | 0 | 5 | 1 | 10 | 0 | 0 | 0 | |
| Low grade dysplasia | 6 | 0 | 2 | 2 | 7 | 0 | 1 | 2 | |
| High grade dysplasia | 2 | 1 | 1 | 6 | 4 | 0 | 0 | 6 | |
| Adenocarcinoma | 3 | 1 | 1 | 5 | 5 | 0 | 0 | 5 | |
Cases italicised and underlined showed complete concordance for p63 and p53 expression.
Scoring of staining pattern: −, no staining observed; +/−, non-nuclear (cytoplasmic or extracellular) staining; +, unequivocal nuclear staining in 10%< x <50% of cells, usually in the proliferative zone of non-neoplastic mucosa; ++, unequivocal nuclear staining of >50% of cells.
IM, intestinal metaplasia.