Reagents used in this study
Antibody | Purpose or localisation of antigen | Dilution | Source |
Anti-H pylori (rabbit polyclonal antibody) | To identify H pylori | 1:10 | Dako |
Antisialosyl-Tn (mouse monoclonal antibody, IgG) | To identify intestinal mucins from metaplastic goblet cells | 1:100 | Dako |
HIK1083 (M-GGMC-1) (mouse monoclonal antibody, IgM) | To identify gastric gland mucous cell mucins | 1:10 | Kanto Chemicals |
Alkaline phosphatase labelled antirabbit immunoglobulin | Second antibody for immunostaining forH pylori | 1:50 | Dako |
Horseradish peroxidase labelled antimouse immunoglobulin | Second antibody for immunostaining for sialosy-Tn | 1:50 | Dako |
Colloidal gold (15 nm) labelled antimouse IgM | Second antibody for electron immunohistochemistry with HIK1083 | 1:20 | EY Laboratories |
Colloidal gold (15 nm) labelled antimouse IgG | Second antibody for electron immunohistochemistry for sialosyl-Tn | 1:20 | EY Laboratories |