Table 1

Protein characteristics of iceA variants

	JHP1132	HP27	Ca1506	1715
Variant	2B	2C	2D	2D'
Amino acids (n)	59	59	94	95
Cysteine (%)^1-151	0	0	0	1
Proline (%)^1-151	0	0	0	0
Topology: N terminus	“in”	“in”	“in”	“in”
Topology: C terminus	?	?	?	?
TopPred
PTM (n)	None	None	One	One
Position^‡(score)			58–78 (0.758)	23–41 (0.615)
DAS
PTM (n)	None	One	Two	Two
Position		32–41	37–40, 66–75	31–41, 70–76
Helix (%)^1-151	11.7	0.0	0.0	0.0
Sheet (%)^1-151	41.7	62.7	72.3	54.2
Other (%)^1-151	46.7	37.3	27.7	45.8
Class^1-151	Mixed	All-beta	All-beta	All-beta

?, not determined; Position^‡, amino acid position;
↵1-151 from JPRED.
“in”, orientation inside cell membrane; PTM, putative transmembrane segment; DAS, dense alignment sequence.