Table 2

Malonaldehyde (MDA) content and aldehyde-protein adducts in homogenates of biopsy tissues from patients affected by colon adenocarcinoma at different stages of malignancy

Tumour stageMDA content (nmol/mg protein)MDA-protein adducts (AFU/mg protein) (390/460 nm ex/em)HNE-protein adducts (AFU/mg protein) (355/460 nm ex/em)
Values are mean (SD) (the number of specimens evaluated is indicated in parentheses).
Stage of tumour malignancy was evaluated following the TNM classification.
Significantly different from normal tissue: *p<0.05, **p<0.005.
Protein-aldehyde adducts were determined as fluorescence exhibited by interaction between protein amino functions and malonaldehyde (MDA) or 4-hydroxynonenal (HNE) at wavelengths of 390/460 nm and 355/460 nm, excitation/emission, respectively.
AFU, arbitrary fluorescence units.
Normal tissue (15)0.67 (0.26)23 (4.0)30 (5.0)
T2 (5)0.52 (0.44)21 (4.0)23 (3.4)*
T3 (8)0.29 (0.17)**16 (5.0)**21 (2.4)**
T4 (2)0.64 (0.17)24 (7.0)27 (4.5)