Sequences of oligonucleotide primers for the PRSS1 gene
| PCR 1 | PCR 2 | Sequencing primer | |
|---|---|---|---|
| PCR, polymerase chain reaction. | |||
| Exon 1 | 5`-GAGTGGCCAAACATAGCCAG-3` | 5`-GAGTGGCCAAACATAGCCAG-3` | |
| 5`-GCATTTGTCGGCCAGGAACG-3` | |||
| Exon 2 | 5`-TGGTCATGGCCAGGTCTATGC-3` | 5`-TTTCTAATTAGCAGAAAGCAAT-3` | 5`-CGCCACCCCTAACATGCTAT-3` |
| 5`-ACAGTTAGCAGAGGTAGAGTG-3` | 5`-CTCTCCCAGGCAGACTGGCC-3` | ||
| Exon 3 | 5`-GGTCCTGGGTCTCATACCTT-3` | 5`-TCCATGAGCAGAGAGCTTGAGGAA-3` | |
| 5`-GTAATGGGCACTCGAAATGT-3` | |||
| Exon 4 | 5`-GACCCACATTTCTACTTCCTTTGATC-3` | 5`-GACCCACATTTCTACTTCCTTTGATC-3` | |
| 5`-CTCAGCATGGGAAGGGTTGG-3` | |||
| Exon 5 | 5`-TATTCCTCCTCCATCTCTCCATAC-3` | 5`-TATTCCTCCTCCATCTCTCCATAC-3` | |
| 5`-AAAGGGGACAGTAGTGTCTAACCTGAA-3` | |||