V_{t} (mV) | R_{t} (Ω×cm^{2}) | V_{a} (mV) | fR_{a} | I_{sc} (μA/cm^{2}) | |
---|---|---|---|---|---|

TNF-α, tumour necrosis factor α; V_{t}, transepithelial potential; V_{a}, intracellular potential; I_{sc}, short circuit current; R_{t}, transepithelial resistance; fR_{a}, fractional resistance. | |||||

Effects of addition of 100 μM histamine to the serosal side of the cells. ΔHistamine represents the difference between phase 2 and baseline. | |||||

n=5 for control experiments; n=7 for TNF-α exposed monolayers except for V_{a} and fR_{a} (n=5). | |||||

Mean incubation time for TNFα (10 ng/ml) was 6–24 hours. | |||||

Statistical significance was evaluated using the Students t test: **p<0.01 compared with control histamine.
| |||||

Control | |||||

Baseline | 1.7 (0.7) | 195 (10) | −62 (6) | 0.67 (0.02) | 9.5 (5) |

Histamine phase 1 | 1.6 (0.7) | 195 (10) | −58 (3) | 0.73 (0.05) | 8.9 (5) |

Histamine phase 2 | 2.5 (0.8) | 190 (11) | −66 (7) | 0.65 (0.02) | 14 (6) |

ΔHistamine | 0.8 (0.1) | −5 (2) | −4 (0.4) | −0.02 (0.01) | 5 (1) |

TNF-α | |||||

Baseline | 2.2 (0.7) | 173 (12) | −57 (2) | 0.70 (0.06) | 13 (4) |

Histamine phase 1 | 2.1 (0.7) | 173 (12) | −54 (3) | 0.73 (0.05) | 12 (4) |

Histamine phase 2 | 8.4 (1)** | 154 (11)** | −38 (2)** | 0.47 (0.07)** | 58 (11)** |

ΔHistamine | 6.2 (0.8)** | −20 (5)** | 19 (3)** | −0.24 (0.05)** | 45 (10)** |