Table 2

Duration of microwaving for antigen retrieval, and concentration of primary antibody with duration of incubation

Monoclonal antibodyMicrowave duration (min)Concentration of primary antibody, duration of incubation
hMLH1301:100 (Pharmingen), 18 h
hMSH2301:100 (Pharmingen), 18 h
β-catenin101:100 (Affiniti), 1 h
E-cadherin20Supernatant (ICRF), 1 h
p53101:1000 (ICRF), 1 h