Table 1

Pretreatment regimens and primary antibody concentrations for immunohistochemistry

Primary antibodyPretreatmentDilution
TIMP, tissue inhibitor of metalloproteinase.
CD3 (monoclonal)Pressure cooker1:200
CD20 (L26)No treatment1:1000
CD68 (PGM-1)Microwave1:200
MIB-1Microwave1:400
Bcl-2Pressure cooker1:200
BaxNone1:50
FasMicrowave1:50
Fas ligandPressure cooker1:80
Retinoblastoma proteinMicrowave1:200
TIMP-1Pronase1:200
TIMP-2Pronase1:400