Effect of TUDC, β-MC, and DBcAMP on the cholestasis induced by TLC
| Control | TLC | TLC+TUDC | TLC+β-MC | TLC+DBcAMP | TLC+TUDC+DBcAMP | |
|---|---|---|---|---|---|---|
| Hepatocyte couplets were incubated for 30 minutes at 37°C with DMSO (controls), TLC, TLC+TUDC, TLC+βMC, TLC+DBcAMP, and TLC+TUDC+DBcAMP. cVA of CLF was then analysed, as described in the methods section. | ||||||
| All values are mean (SD) (n=5–6). | ||||||
| TUDC, β-MC, and DBcAMP had a significant protective effect against TLC induced cholestasis. No additive protective effect was observed when both TUDC and DBcAMP where administered together. | ||||||
| Significantly different from TLC, **p<0.01, ***p<0.001. | ||||||
| CLF, cholyl-lysyl-fluorescein; DBcAMP (dibutyryl cAMP), N6,2`-o-dibutyryladenosine 3`:5`-cyclic monophosphate; DMSO, dimethyl sulphoxide; cVA, canalicular vacuolar accumulation; β-MC, β-muricholate; TLC, taurolithocholate; TUDC, tauroursodeoxycholate. | ||||||
| cVA of CLF (%) | 57 (3) | 25 (7) | 41 (9)*** | 42 (5)*** | 41 (13)*** | 36 (11)** |