Table 1

Sequences of oligonucleotides used as polymerase chain reaction primers

Oligonucleotide primersSequence (5′ to 3′)
*External 5′ primers hybridise to the 5′ leader sequence.
†Internal 5′ primers hybridise to the framework region 1.
Amplification of IgVHDJH-CH transcripts
    External 5′ primers*
        VH1-leaderCCATGGACTGGACCTGGA
        VH3-leaderCCATGGAGTTTGGGCTGAGC
        VH5-leaderATGGGGTCAACCGCCATCCT
    Internal 5′ primers†
        VH1-FR1CCTCAGTGAAGGTCTCCTGCAAGG
        VH3-FR1GGTCCCTGAGACTCTCCTGTGCA
        VH5-FR1GAAAAAGCCCGGGGAGTCTCTGAA
    3′ CH primers
        CμCAGGAGACGAGGGGGAA
        CαGGAAGAAGCCCTGGACCAGGC
        CγCACCGTCACCGGTTCGG
IgG clone CDR3 specific primers
    Clone 1, patient No 1CTAAGAAGTAGCAGCACCTG
    Clone 2, patient No 1GCGCCTTTACCCCTACTATTCG
    Clone 3, patient No 1CGTCGGGAGGGAGCTTCTATG
    Clone 4, patient No 1GGAAGCAGACACTATGG
    Clone 5, patient No 2GAGGTGGGAGGACTATAGC
    Clone 6, patient No 2CGCGTAGGGGCGGTCTGGC
    Clone 7, patient No 2CGGGTACAGCAGTGGC