Distribution of IgG clones and related IgA switch variants using CDR3 clone specific primers
| Distribution of cells related to IgG clones 1–7 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient No 1 | Patient No 2† | ||||||||||
| IgG-clone 1 | IgG-clone 2 | IgG-clone 3 | IgG-clone 4 | ||||||||
| IgG | IgA | IgG | IgA | IgG | IgA | IgG | IgA | IgG-clone 5 | IgG-clone 6 | IgG-clone 7 | |
| *Sample from which the original IgG sequences were derived. | |||||||||||
| †No IgA+ cells related to IgG were detected by this method in patient No 2. | |||||||||||
| +, strong unambiguous band; +/−, weak band. | |||||||||||
| NT, no tissue: two blocks of each of normal and diseased mucosa only for patient No 1. | |||||||||||
| Sample | |||||||||||
| Diseased colon | |||||||||||
| 1* | + | + | + | − | + | − | + | − | + | + | + |
| 2 | + | + | + | + | + | + | + | + | + | + | + |
| 3 | NT | NT | NT | NT | NT | NT | NT | NT | + | + | + |
| 4 | NT | NT | NT | NT | NT | NT | NT | NT | + | + | + |
| Normal mucosa | |||||||||||
| 1 | − | − | + | − | − | − | − | − | − | − | − |
| 2 | +/− | − | − | + | +/− | − | +/− | − | − | − | − |
| 3 | NT | NT | NT | NT | NT | NT | NT | NT | − | − | − |
| 4 | NT | NT | NT | NT | NT | NT | NT | NT | − | − | − |
| PBL | + | + | + | − | +/− | + | − | +/− | + | + | − |