Table 1

Primer pairs used to quantify iron related transcripts expressed in the duodenum

GeneSense primerAntisense primerGenBank accession No (position)
Dcytb, duodenal cytochrome b; DMT1, divalent metal transporter 1; SFT, stimulator of Fe transport; FPN1, ferroportin 1; TfR1, transferrin receptor 1.
Dcytb5′-GCAGCGGGCTCGAGTTTA-3′5′-TTCCAGGTCCATGGCAGTCT-3′AF354666 (137–235)
DMT15′-GGCTTTCTTATGAGCATTGCCTA-3′5′-GGAGCACCCAGAGCAGCTTA-3′L33415 (289–385)
SFT5′-CTGTGCTCATTGAAGAGGACCTT-3′5′-TCTGGTTGCTTTCTCAGTCACG-3′AA178012 (232–329)
FPN15′-TTGCAGGAGTCATTGCTGCTA-3′5′-TGGAGTTCTGCACACCATTGAT-3′AF226613 (1670–1789)
Hephaestin5′-TTGTCTCATGAAGAACATTACAGCAC-3′5′-CATATGGCAATCAAAGCAGAAGA-3′AF082567 (3803–3963)
HFE5′-CTGAAAGGGTGGGACTACATGTTC-3′5′-GGACACCACTCCCAACTTCGT-3′U66849 (362–454)
TfR15′-TCATGAGGGAAATCAATGATCGTA-3′5′-GCCCCAGAAGATATGTCGGAA-3′X57349 (2018–2118)
β-Actin5′-GACGGCCAAGTCATCACTATTG-3′5′-CCACAGGATTCCATACCCAAGA-3′M12481 (652–740)