Gene array analysis and in situ hybridisation of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) genes in day 4 human fetal gut explant cultures
| Gene array analysis | In situ hybridisation* | |||||
|---|---|---|---|---|---|---|
| MMP | PWM (fold change v controls) | αCD3+IL-12 (fold change v controls) | Performed in this study | Control | PWM | αCD3+IL-12 |
| The gene arrays detected MMP and TIMP genes in day 4 cultured explants, the fold change over control were quantified by computer. | ||||||
| *In situ hybridisation signal was evaluated using dark field microscopy. | ||||||
| Signal strength was assessed as follows: −, no detectable specific signal; +, specific signal in few cells; ++, specific signal in moderate number of cells; +++, specific signal in a high number of cells | ||||||
| †In situ hybridisation data were shown in our previous study.17 | ||||||
| ND, not determined; PWM, pokeweed mitogen; IL-12, interleukin-12. | ||||||
| Collagenase 1 (MMP-1)† | 3.8 | 2.6 | Yes | − | +++ | + |
| Gelatinase A (MMP-2) | −1.1 | 1.6 | No | ND | ND | ND |
| Stromelysin 1 (MMP-3)† | 8.2 | 5.7 | Yes | + | +++ | ++ |
| Matrilysin (MMP-7) | −1.9 | −2.0 | Yes | − | ++ | + |
| Neutrophil collagenase (MMP-8) | −1.4 | 1.0 | No | ND | ND | ND |
| Gelatinase B (MMP-9) | 6.2 | 5.5 | No | ND | ND | ND |
| Stromelysin 2 (MMP-10) | 4.0 | 2.6 | Yes | − | +++ | ++ |
| Stromelysin 3 (MMP-11) | −1.1 | 1.1 | No | ND | ND | ND |
| Macrophage metalloelastase (MMP-12) | 11.9 | 7.3 | Yes | + | +++ | ++ |
| Collagenase 3 (MMP13) | 1.2 | 1.5 | Yes | − | − | + |
| MT1-MMP (MMP-14) | −1.0 | −1.2 | Yes | + | + | + |
| MT2-MMP (MMP-15) | −1.4 | −1.0 | No | ND | ND | ND |
| MT3-MMP (MMP-16) | −1.7 | −1.4 | No | ND | ND | ND |
| MT4-MMP (MMP-17) | 1.7 | 2.7 | No | ND | ND | ND |
| MMP-19 | −1.2 | 1.1 | No | ND | ND | ND |
| TIMP-1 | −1.4 | −1.1 | Yes | +++ | + | + |
| TIMP-2 | −1.0 | −1.1 | No | ND | ND | ND |
| TIMP-3 | 1.5 | 1.1 | Yes | +++ | + | + |
| TIMP-4 | 1.7 | 1.1 | No | ND | ND | ND |