Primer pairs for each exon of the 5β-reductase gene and the primer used to generate an amplimer suitable for restriction enzyme digest in patient MS
| Exon 1 | Forward | ggtgtgttaatctcccttccc |
| Backward | aagaaacaaacaagcaagcaaag | |
| Exon 2 | Forward | tttctcttttccccaaaccc |
| Backward | cacacacacacacacggaag | |
| Exon 3 | Forward | gggctgccttatcgtaaaaa |
| Backward | acctttgggcatctgaactc | |
| Exon 4 | Forward | gctcacaattatgaagactggg |
| Backward | tgaaagtaaagggtgcatctctc | |
| Exon 5 | Forward | ggcttgtttgttgacctgtg |
| Backward | tcatgagccttcctgactcc | |
| Exon 6 | Forward | ttgtgctctgctctccaatg |
| Backward | accaaaaggcattccaatca | |
| Exon 7 | Forward | gtgactgggtgaatgggaag |
| Backward | ttcacatctccaaaagcaattc | |
| Exon 8 | Forward | tgatcccaagcttgaccttc |
| Backward | gactacgttgctagctagc | |
| Exon 9 | Forward | tctgtgggggaattgttttg |
| Backward | tgagtcaaagtcatttacactgcac | |
| MS RE product | Forward | tgttcttgaatccattttaaggaagc |
| Backward | cctttggggaccagtaggaatc |