Peptide mapping of MODE K derived epithelial exosomes using matrix assisted laser desorption ionisation-time of flight-mass spectrometry (MALDI-TOF-MS) analysis
| Band No | Proteins identified from MODE K derived exosomes | Molecular mass (kDa) | Proteins identified from MODE K+IFN-γ derived exosomes | Molecular mass (kDa) |
|---|---|---|---|---|
| MODE K derived exosomes (20 µg) were run on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis and stained with Coomassie blue. The major bands were excised from the gel and in-gel digested with trypsin. The digest solution was analysed by MALDI-TOF-MS. | ||||
| IFN-γ, interferon γ. | ||||
| Band M-1 | A-X actin, Q61276 | 41.7 | ||
| Clathrin heavy chain P11442 | 193.2 | |||
| Similar to ubiquitin C P11276 Q922Z8 65.5kD | 273.3 | |||
| Fibronectin precursor P11276 | 273.3 | |||
| Heat shock protein HSP 90-alpha P07901 | 85.5 | |||
| Heat shock protein HSP 71 kD P08109 | 71 | Heat shock protein HSP 71 kD P08109 | 71 | |
| Heat shock protein HSP 90-beta protein P34058 | Heat shock protein HSP 90-beta protein P34058 | 85.5 | ||
| Band M-2 | Similar to ubiquitin C, Q922Z8 | 65.5 | ||
| Band M-3 | Integrin beta-1 precursor, P09055 | 91.7 | Integrin beta-1 precursor, P09055 | 91.7 |
| Integrin alpha-3 precursor, Q62470 | 117.8 | |||
| Band M-4 | Prostaglandin F2-alpha receptor regulatory protein, Q9WV91 | 99.6 | Prostaglandin F2-alpha receptor regulatory protein, Q9WV91 | 99.6 |
| Band M-5 | Serine protease HTRA1 precursor, Q9R118 | 51.2 | Moesin, P26041 | 67.6 |
| Band M-6 | Moesin, P26041 | 67.6 | ||
| Band M-7 | EH-domain containing protein 1, Q9WVK4 | 60.6 | ||
| Band M-8 | Pyruvate kinase, M2 isozyme, P52480 | 57.7 | ||
| Band M-9 | 22 kDa Neuronal tissue-enriched acidic protein., Q91XV3 | 22.2 | 22 kDa Neuronal tissue enriched acidic protein, Q91XV3 | 22.2 |
| Pyruvate kinase, P52480 | 57.7 | |||
| CD82 antigen, P40237 | 29.6 | CD82 antigen, P40237 | 29.6 | |
| Band M-10 | Milk fat globule glycoprotein MFG-E8 S | 48.8 | Alpha Enolase, P17182 | 47.3 |
| Calpactin I light chain, P08207 | 11.1 | Annexin II, P07356 | 38.8 | |
| Band M-11 | Annexin I P10107 | 38.8 | H-2 class I histocompatibility antigen, P04223 | 41.6 |
| Kératine P02535 | 57.8 | |||
| Band M-12 | Annexin A4 (Annexin IV), P97429 | 36.1 | ||
| Annexin I (Lipocortin I) P10107 | 38.8 | |||
| Band M-13 | Transmembrane 4 superfamily, Q64302 | 23.1 | ||
| Annexin I, P10107 | 38.8 | |||
| Band M-14 | Annexin V, P48036 | 35.7 | CD9 antigen, P40240 | 25.0 |
| Annexin I, P10107 | 38.5 | CD81 antigen, P35762 | 25.8 | |
| Annexin A4, P97429 | 35,8 | |||
| Histone H1.3, P15864 | 22.2 | |||
| Histone H1.5, P43276 | 22.4 | |||
| Histone H1.4, P43274 | 21.8 | |||
| Band M-15 | Annexin A4, P97429 | 35.8 | ||
| Annexin I, P10107 | 38.7 | |||
| Band M-16 | Histone H3, P02301 | 14.9 | 14-3-3 protein zeta/delta, P35215 | 27.7 |
| Histone H1.1, P15864 | 21.1 | |||
| Band M-17 | Ferritin light chain 1, P29391 | 20.6 | ||
| Ras related protein Rab-2, P53994 | 23.5 | |||
| Ras related protein Rab-7, P51150 | 23.5 | |||
| Myeloid associated differentiation marker, O35682 | 32.08 | |||
| Band M-18 | Histone H1.3, P43277 | 21.9 | ||
| Band M-19 | Histone H2B F, P10853 | 13.8 | ||
| Ferritin heavy chain, P09528 | 21.0 | |||