Amino acid sequences of synthetic peptides and substituted peptides used in this study (single letter code)
| *Residues 56–68 of α-9 gliadin was synthesised, compared with the sequence published.6 This was because the synthetic sequence 57–68 was found to be unstable at the N terminal glutamine residue, which was partially cyclised forming pyroglutamic acid. | |
| Amino acid sequences of synthetic peptides | |
| PQPELPYPQPQLPY (α-2 gliadin, residues 62–75, E65) | G4 |
| LQLQPFPQPELPY* (α-9 gliadin, residues 56–68, E65) | G5 |
| LQLQPFPQPQLPYPQPQLPY (α-2 gliadin, residues 56–75) | G8 |
| LQLQPFPQPELPYPQPQLPY (α-2 gliadin, residues 56–75, E65) | G9 |
| Amino acid substituted peptides | |
| PQPELPYPQPQLPY (G4) | |
| AQPELPYPQPQLPY (G4-1A) | |
| PAPELPYPQPQLPY (G4-2A) | |
| PQAELPYPQPQLPY (G-3A) | |
| PQPEAPYPQPQLPY (G-5A) | |
| PQPELAYPQPQLPY (G-6A) | |
| PQPELPAPQPQLPY (G-7A) | |
| PQPELPYAQPQLPY (G4-8A) | |
| PQPELPYPAPQLPY (G4-9A) | |
| PQPELPYPQAQLPY (G4-10A) | |
| PQPELPYPQPALPY (G4-11A) | |
| PQPELPYPQPQAPY (G4-12A) | |
| PQPELPYPQPQLAY (G4-13A) | |
| PQPELPYPQPQLPA (G4-14A) | |