Amino acid sequences of synthetic peptides and substituted peptides used in this study (single letter code)
*Residues 56–68 of α-9 gliadin was synthesised, compared with the sequence published.6 This was because the synthetic sequence 57–68 was found to be unstable at the N terminal glutamine residue, which was partially cyclised forming pyroglutamic acid. | |
Amino acid sequences of synthetic peptides | |
PQPELPYPQPQLPY (α-2 gliadin, residues 62–75, E65) | G4 |
LQLQPFPQPELPY* (α-9 gliadin, residues 56–68, E65) | G5 |
LQLQPFPQPQLPYPQPQLPY (α-2 gliadin, residues 56–75) | G8 |
LQLQPFPQPELPYPQPQLPY (α-2 gliadin, residues 56–75, E65) | G9 |
Amino acid substituted peptides | |
PQPELPYPQPQLPY (G4) | |
AQPELPYPQPQLPY (G4-1A) | |
PAPELPYPQPQLPY (G4-2A) | |
PQAELPYPQPQLPY (G-3A) | |
PQPEAPYPQPQLPY (G-5A) | |
PQPELAYPQPQLPY (G-6A) | |
PQPELPAPQPQLPY (G-7A) | |
PQPELPYAQPQLPY (G4-8A) | |
PQPELPYPAPQLPY (G4-9A) | |
PQPELPYPQAQLPY (G4-10A) | |
PQPELPYPQPALPY (G4-11A) | |
PQPELPYPQPQAPY (G4-12A) | |
PQPELPYPQPQLAY (G4-13A) | |
PQPELPYPQPQLPA (G4-14A) |