Quantitative bacteriology of caecal content by fluorescent in situ hybridisation
| Treatment | Total bacterial count (108) | E coli (107) | E faecalis (107) | Bacteroides spp (107) | Lactobacilli spp (107) |
|---|---|---|---|---|---|
| Caecal luminal concentrations of Escherichia coli, enterococci, Bacteroides spp, and lactobacilli spp in gnotobiotic IL-10−/− mice colonised with specific pathogen free flora and treated with ciprofloxacin (Cipro), metronidazole (MNZ), or vancomycin-imipenem (V+I). Values are given as mean (SEM) per gram of caecal content, measured by fluorescent in situ hybridisation using probes specific for 16S ribosomal RNA of E coli, Enterococcus faecalis, Bacteroides spp, and lactobacilli spp. | |||||
| <1, below detection limit of 107 bacteria/g content. | |||||
| **p<0.01 versus water controls. | |||||
| Water | 86 (21) | 6 (2) | 40 (38) | 114 (39) | 141 (40) |
| MNZ | 85 (9) | 171 (47)** | 78 (40) | <1** | 247 (48) |
| Cipro | 31 (7) | <1** | <1** | 61 (12) | 11 (5)** |
| V+I | 18 (1)** | <1** | <1** | <1** | <1** |