Reactivity of clones 6 and 8 with alanine substituted analogues of peptide G4
| Test (clone 6) | CPM | SI | IFN-γ | IL-4 |
|---|---|---|---|---|
| Values are mean (SEM). | ||||
| CPM, counts per minute; APC, antigen presenting cells; SI, stimulation index. | ||||
| Interferon γ (IFN-γ) and interleukin 4 (IL-4) concentrations shown in pg/ml. Limits of sensitivity: IL-4 30 pg/ml, IFN-γ 30 pg/ml. | ||||
| T+APC only | 572 (42) | — | 0 | 0 |
| PQPELPYPQPQLPY | 9528 (124) | 15 | 400 | 0 |
| AQPELPYPQPQLPY | 1086 (211) | 2 | 60 | 0 |
| PAPELPYPQPQLPY | 590 (48) | 1 | 42 | 0 |
| Test (clone 6) | CPM | SI | IFN-γ | IL-4 |
| T+APC only | 1053 (911) | — | 31 | 0 |
| PQPELPYPQPQLPY | 14764 (3870) | 14 | 232 | 0 |
| PQAELPYPQPQLPY | 343 (110) | <1 | 0 | 0 |
| PQPEAPYPQPQLPY | 244 (59) | <1 | 0 | 0 |
| PQPELAYPQPQLPY | 182 (42) | <1 | 0 | 0 |
| PQPELPAPQPQLPY | 236 (25) | <1 | 0 | 0 |
| PQPELPYAQPQLPY | 211 (33) | <1 | 0 | 0 |
| PQPELPYPAPQLPY | 798 (123) | <1 | 31 | 0 |
| PQPELPYPQAQLPY | 341 (70) | <1 | 0 | 0 |
| PQPELPYPQPALPY | 14454 (197) | 14 | 205 | 0 |
| PQPELPYPQPQAPY | 13681 (1209) | 13 | 225 | 0 |
| Test (clone 8) | CPM | SI | IFN-γ | IL-4 |
| T+APC | 4238 (418) | — | 50 | 0 |
| PQPELPYPQPQLPY | 51683 (913) | 12 | 575 | 130 |
| PQPELPYPQPQLAY | 54782 (771) | 13 | 600 | nd |
| PQPELPYPQPQLPA | 57467 (1999) | 14 | 600 | nd |