Cytokine primer and probe sequences
| Primers and probes | Sequence | Amplicon |
|---|---|---|
| IFN-γ, interferon γ; IL, interleukin. | ||
| IFN-γ | ||
| Probe | 5` TGTCACTCTCCTCTTTCCAATTCTTCAAAATGC 3` | 96 bp |
| Forward primer | 5` TTCAGATGTAGCGGATAATGGAAC 3` | |
| Reverse primer | 5` GAGACAATTTGGCTCTGCATTATTT 3` | |
| Il-2 | ||
| Probe | 5`ATGCTCACATTTAAGTTTTACATGCCCAA 3` | 88 bp |
| Forward primer | 5` GAATTAATAATTACAAGAATCCCAAACT 3` | |
| Reverse primer | 5` AGATGTTTCAGTTCTGTGGCC 3` | |