Table 1

Effect of incubation media on markers of monolayer integrity and cellular ATP content

Experimental mediumLY (pmol/cm2/4 h)TEER (Ω/cm2)LDH (%)ATP concentration (pmol/μg protein)
All data are mean (SD); n=6.
Gln, glutamine; ConGln, DMEM containing glutamine; GlnF, glutamine free DMEM; TNF, tumour necrosis factor α; AOA, aminooxyacetate; AKG, α-ketoglutarate; TEER, transepithelial electrical resistance; LY, lucifer yellow; LDH, lactate dehydrogenase.
**p<0.01 versus ConGln; ††p<0.01 versus ConGln+TNF+AOA.
ConGln287.9 (65.6)215.0 (8.9)6.5 (1.3)197.3 (11.1)
GlnF317.2 (53.7)209.2 (7.4)7.4 (2.2)190.0 (30.5)
ConGln+TNF308.1 (78.4)210.0 (8.9)7.1 (2.9)177.7 (14.6)
GlnF+TNF322.4 (42.8)208.3 (9.8)6.8 (1.4)104.3 (17.8)**
GlnF+TNF+Gln290.8 (65.3)216.3 (18.0)183.7 (15.7)
ConGln+TNF+AOA306.0 (30.3)211.2 (6.2) 99.3 (32.8)**
ConGln+TNF+AOA+Gln292.3 (45.9)196.5 (14.4)151.3 (13.3)††
ConGln+TNF+AOA+AKG314.0 (52.6)200.0 (12.9)181.7 (22.8)