Effect of incubation media on markers of monolayer integrity and cellular ATP content
| Experimental medium | LY (pmol/cm2/4 h) | TEER (Ω/cm2) | LDH (%) | ATP concentration (pmol/μg protein) |
|---|---|---|---|---|
| All data are mean (SD); n=6. | ||||
| Gln, glutamine; ConGln, DMEM containing glutamine; GlnF, glutamine free DMEM; TNF, tumour necrosis factor α; AOA, aminooxyacetate; AKG, α-ketoglutarate; TEER, transepithelial electrical resistance; LY, lucifer yellow; LDH, lactate dehydrogenase. | ||||
| **p<0.01 versus ConGln; ††p<0.01 versus ConGln+TNF+AOA. | ||||
| ConGln | 287.9 (65.6) | 215.0 (8.9) | 6.5 (1.3) | 197.3 (11.1) |
| GlnF | 317.2 (53.7) | 209.2 (7.4) | 7.4 (2.2) | 190.0 (30.5) |
| ConGln+TNF | 308.1 (78.4) | 210.0 (8.9) | 7.1 (2.9) | 177.7 (14.6) |
| GlnF+TNF | 322.4 (42.8) | 208.3 (9.8) | 6.8 (1.4) | 104.3 (17.8)** |
| GlnF+TNF+Gln | 290.8 (65.3) | 216.3 (18.0) | — | 183.7 (15.7) |
| ConGln+TNF+AOA | 306.0 (30.3) | 211.2 (6.2) | — | 99.3 (32.8)** |
| ConGln+TNF+AOA+Gln | 292.3 (45.9) | 196.5 (14.4) | — | 151.3 (13.3)†† |
| ConGln+TNF+AOA+AKG | 314.0 (52.6) | 200.0 (12.9) | — | 181.7 (22.8) |