Concentrations of organic acids in broth with and without Fusobacterium varium isolated from colonic mucosa of patients with ulcerative colitis
| Mean concentration (mM) in: | |||||
|---|---|---|---|---|---|
| Organic acid | Culture (n=5) | ABCM broth only (n=2) | Well of Vero cell culture† [pH] | Cytotoxicity to Vero cell | Lowest concentration for cytotoxicity† [pH] |
| *Results are means (SD). | |||||
| †Fifthfold diluted concentration of organic acids in cultured broth with Fusobacterium varium. | |||||
| ND, not detected | |||||
| Maleic acid | 0.108 (0.011)* | 0.188 (0.019) | 0.022 [7.87 (0.18)] | − | 5.0 [6.30 (0.08)] |
| Succinic acid | 7.51 (0.93) | 1.04 (0.28) | 1.50 [7.16 (0.11)] | − | 6.5 [5.77 (0.08)] |
| Lactic acid | 16.3 (5.90) | 9.99 (1.23) | 3.26 [7.27 (0.14)] | − | 12 [6.24 (0.10)] |
| Formic acid | 1.17 (0.37) | 1.28 (0.05) | 0.234 [7.84 (0.17)] | − | 5.0 [7.03 (0.10)] |
| Acetic acid | 26.5 (3.5) | 4.49 (0.22) | 5.30 [6.93 (0.13)] | − | 17 [5.02 (0.09)] |
| Propionic acid | 7.13 (0.56) | ND | 1.43 [7.52 (0.23)] | − | 5.0 [7.02 (0.08)] |
| n-Butyric acid | 32.1 (3.9) | ND | 6.42 [6.90 (0.15)] | + | 1.5 [7.65 (0.10)] |
| Total | 90.8 (4.6) | 17.0 (1.1) | |||