Primers used for reverse transcription-polymerase chain reaction (RT-PCR), minisequencing, and direct sequencing
| PCR primers for minisequencing | |||
|---|---|---|---|
| Exon | Reverse primer | Forward primer | Product (bp) |
| SNP, single nucleotide polymorphism. | |||
| 1 | 5′-AGATATGGGTGGTTCTAGCAG | 5′-(biotin)ACCTAGTTGGGATCTGGTTCA | 217 |
| 2 | 5′-AGATATGGGTGGTTCTAGCAG | 5′-(biotin)ACCTAGTTGGGATCTGGTTCA | 217 |
| 6 | 5′-TCAATCAGCTTGTTGTAGTAGG | 5′-(biotin)CTCTGCCTATCAGAGAATCTG | 378 |
| 10 | 5′-ATGAGCCTCACGTAGTAGTTC | 5′-(biotin)GCAGCTTCTGCTGCATATCAG | 278 |
| 10 | 5′-ATGAGCCTCACGTAGTAGTTC | 5′-(biotin)GCAGCTTCTGCTGCATATCAG | 278 |
| 13 | 5′-ACAGTGGTGTAGTGATTGAAC | 5′-(biotin)AGGAGATATGTTCAGTTCATG | 206 |
| 17 | 5′-(biotin)AAATGCCAAGCCACAGACTCC | 5′-CATCAGCGAAGTTCTACGCCT | 224 |
| Detection primers for minisequencing | SNP | ||
| 1 | 5′-TTTTCTGTGGGCATCACTGAG | GA | |
| 2 | 5′-TATCTTCAGCTCGCAGGACAA | CT | |
| 6 | 5′-AGCGTCGCTTGGCCCTCA | GT | |
| 10 | det1: 5′-TGCGAGACCAGGAGATGGA | GA | |
| 10 | det2: 5′-GAAAAACGGTAGTGGGACAC | GA | |
| 13 | 5′-ATCCGCGTCTTCATCACCTCA | CT | |
| 17 | 5′-TTGTCTCCACCAGCCAGATGC | CT | |
| Sequencing primers | Product (bp) | ||
| Reverse primer | Forward primer | Product (bp) | |
| 1 | 5′-TGTCGAATCTGCTCTAAGGAG | 5′-ACCTAGTTGGGATCTGGTTCA | 232 |
| 2 | 5′-CTCTCCTCAGATGTTACAGGT | 5′-TCACCTGCTGTTCGTGTCTCT | 155 |
| 6 | 5′-CAGCCAATGTCCTTTCTCCTCCAA | 5′-TAAACACAGTAGTCATCCGATGGG | 860 |
| 10 | 5′-TGCTGTTCCTGCATGAGGAC | 5′-CGTCCTGACTTGAGACTCAC | 433 |
| 13 | 5′-GCTCAGTCATGGTAACTTGC | 5′-TGGACACTGAAGCTGCAACA | 360 |
| 17 | 5′-AAATGCCAAGCCACAGACTCC | 5′-CTTCACTCAGAGGCTCCTGAC | 309 |