Experimental design for the checkerboard assay
| Upper chamber | |||
|---|---|---|---|
| 0 ng/ml PDGF | 1 ng/ml PDGF | 10 ng/ml PDGF | |
| Varying concentrations of platelet derived growth factor (PDGF) were introduced into the upper and lower chambers of the insert assembly and migration of pancreatic stellate cells assessed after nine hours of incubation in the absence or presence of a concentration gradient of PDGF in the culture medium. Each cell of the table denotes the concentrations of PDGF initially established in the upper and lower chambers of the insert assembly. | |||
| Lower chamber | |||
| 0 ng/ml PDGF | 0/0 | 1/0 | 10/0 |
| 1 ng/ml PDGF | 0/1 | 1/1 | 10/1 |
| 10 ng/ml PDGF | 0/10 | 1/10 | 10/10 |