Table 2

Permeability of HT29/cl.19A and Caco-2 cells after treatment with EIEC and ST/LA in vitro

Clearance (nl/cm2/h)
Bacteria†/probeHT29/cl.19ACaco-2
†Bacteria MOI (multiplicity of infection)=50:1; permeability assays were performed at 12 hours after infection; experiments were performed three times in triplicate.
‡Pretreatment with ST/LA followed by EIEC infection.
‡Simultaneous inoculation of the three strains.
§EIEC infection followed by ST/LA treatment.
*p<0.05, **p<0.01, ***p<0.001, clearance values significantly different from corresponding control values (by ANOVA).
ST/LA, Streptococcus thermophilus and Lactobacillus acidophilus; EIEC, enteroinvasive Escherichia coli; FS, fluorescein sulphonic acid; FITC-D10, fluorescein isothiocyanate-dextran-10S.
Control
    FS395 (88)532 (110)
    FITC-D1098 (22)126 (35)
S thermophilus
    FS276 (33)321 (66)*
    FITC-D1082 (15)89 (34)
L acidophilus
    FS245 (14)*299 (30)*
    FITC-D1080 (9)91 (21)
ST/LA
    FS214 (21)*236 (26)**
    FITC-D1078 (11)84 (9)
EIEC
    FS16590 (3318)***17775 (4050)***
    FITC-D103010 (1185)***3826 (957)***
ST/LA+EIEC†
    FS408 (125)453 (99)
    FITC-D10123 (36)155 (37)
ST/LA/EIEC‡
    FS13872 (2295)***16358 (3922)***
    FITC-D104973 (1104)***3349 (1003)***
EIEC (ST/LA)§
    FS18529 (4185)***16554 (2939)***
    FITC-D104666 (1010)***3935 (1230)***