Table 3

Effect of ST/LA on adhesion† of EIEC to Caco-2 cell monolayers

Time after EIEC addition (h)
Probiotic treatmentRatio of MOI‡ (ST/LA:EIEC)123Significance
†Mean (SEM) of adherent EIEC per 100 cells examined in 20 random microscopic fields, for four experiments in duplicate.
‡MOI=multiplicity of infection. EIEC was applied at 50:1 throughout.
§ST/LA spent medium from 48 hour cultures was filtered, lyophilised, and concentrated 10×.
*p<0.05, **p<0.01, ***p<0.001, data sets significantly different from EIEC with no probiotics (by repeated measures ANOVA).
ST/LA, Streptococcus thermophilus and Lactobacillus acidophilus; EIEC, enteroinvasive Escherichia coli.
NoneN/A24 (3)27 (1)17 (5)
Live ST/LA 1 h prior to EIEC2:16 (2)5 (1)5 (2)**
20:12 (1)1 (1)0***
200:11 (1)00***
Live ST/LA simultaneous with EIEC2:119 (4)21 (4)24 (3)NS
20:115 (3)17 (2)11 (5)NS
200:15 (1)7 (3)5 (1)**
Live ST/LA 1 h after EIEC2:122 (5)29 (3)25 (2)NS
20:120 (4)23 (1)22 (3)NS
200:121 (5)22 (2)23 (1)NS
ST/LA (antibiotic killed) 1 h prior to EIEC2:110 (2)9 (1)10 (1)**
20:17 (1)7 (1)8 (1)**
200:13 (1)3 (2)3 (1)**
ST/LA (antibiotic killed) simultaneous with EIEC2:118 (2)19 (1)18 (1)*
20:116 (1)16 (2)15 (1)*
200:15 (1)7 (2)5 (1)**
ST/LA (heat inactivated) 1 h prior to EIEC2:122 (1)25 (2)23 (2)NS
20:120 (1)23 (1)20 (1)NS
200:121 (2)20 (2)20 (1)NS
ST/LA sp medium§ simultaneous with EIEC25 (2)23 (2)22 (1)NS