Primers and probes, and selected polymerase chain reaction (PCR) conditions
| PCR product | Temperature (°C)* | Primer sequence (5′→3′) |
|---|---|---|
| *Annealing temperature. | ||
| MIP, macrophage inflammatory protein; IL, interleukin; IFN-γ, interferon γ; MCP-1, monocyte chemoattractant protein 1; COX-2, cyclooxygenase 2. | ||
| MIP-1α | ||
| Sense | 58 | 5′-TCA CCT GCT CAG AAT CAT GC-3′ |
| Antisense | 5′-TCC ATA GAA GAG GTA GCT GTG G-3′ | |
| MIP-1β | ||
| Sense | 57 | 5′-AGC AGC TCA GTT CAG TTC CAG GTC A-3′ |
| Antisense | 5′-CCA TGA AGC TCT GCG TGA CTG TCC T-3′ | |
| IP-10 | ||
| Sense | 57 | 5′-ACC ATG AAC CCA AGT GCT GCC GTC-3′ |
| Antisense | 5′-GCT TCA CTC CAG TTA AGG AGC CCT-3′ | |
| IL-7 | ||
| Sense | 60 | 5′-ATG TTC CAT GTT TCT TTT AGG TAT ATC T-3′ |
| Antisense | 5′-TGC ATT TCT CAA ATG CCC TAA TCC G-3′ | |
| IFN-γ | ||
| Sense | 57 | 5′-GGA CCC ATA TGT AAA AGA AGC AGA-3′ |
| Antisense | 5′-TGT CAC TCT CCT CTT TCC AAT TCT-3′ | |
| IL-8 | ||
| Sense | 65 | 5′-AAG GAA CCA TCT CAC TG-3′ |
| Antisense | 5′-GAT TCT TGG ATA CCA CAG AG-3′ | |
| MCP-1 | ||
| Sense | 57 | 5′-TCC TGT GCC TGC TGC TCA TAG C-3′ |
| Antisense | 5′-TTC TGA ACC CAC TTC TGC TTG G-3′ | |
| RANTES | ||
| Sense | 65 | 5′-ATG AAG GTC TCC GCG GCA CGC-3′ |
| Antisense | 5′-CTA GCT CAT CTC CAA AGA GTT-3′ | |
| COX-2 | ||
| Forward | 60 | 5′-GCCAGAGTTTCACCGTAAATATGATT 3′ |
| Reverse | 5′-GCGAGGGCCAGCTTTCA-3′ | |
| Fluorogenic probe | 5′-Fam-AAGTCCACCCCATGGCCCAGC-Tamra-3′ | |
| β-actin | ||
| Forward | 60 | 5′-TCACCCACACTGTGCCCATCTACGA 3′ |
| Reverse | 5′-CAGCGGAACCGCTCATTGCCAATGG-3′ | |
| Fluorogenic probe | 5′-Fam-ATGCCC-Tamura-CCCCCATGCCATCCTGCGT-3′ | |